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  • Title: Cloning of cDNAs coding for heavy- and light-chain variable regions of a murine monoclonal antibody with specificity for human plasma apolipoprotein A-I.
    Author: Lee SH, Kim CS, Cho MH.
    Journal: Res Commun Mol Pathol Pharmacol; 1997 Sep; 97(3):263-77. PubMed ID: 9387187.
    Abstract:
    We prepared a monoclonal antibody (mAb) A-I4-48 to HDL apolipoprotein A-I with the ultimate goals of expressing the valuable immunodiagnostic single-chain Fv (scFv) in Escherichia coli. The binding specificity of mAb A-I4-48 was determined by ELISA and Western blot analysis. The dissociation constant (Kd) of antigen-antibody complex obtained by ELISA was 8.33 x 10(-8) M. Poly(A)+ RNA was prepared from the hybridoma cell line secreting mAb A-I4-48 and used as a template for cDNA synthesis and cloning. The nucleotide sequence analyses revealed that the variable regions of the heavy- and light-chains were members of mouse heavy-chain subgroup IIA and kappa light-chain subgroup II, respectively. Comparison of the nucleotide sequences with mouse immunoglobulin genes in the GenBank data base showed that the cDNAs have not been previously reported.
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