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  • Title: Self-splicing activity of the mitochondrial group-I introns from Aspergillus nidulans and related introns from other species.
    Author: Hur M, Geese WJ, Waring RB.
    Journal: Curr Genet; 1997 Dec; 32(6):399-407. PubMed ID: 9388295.
    Abstract:
    The mitochondrial genome of Aspergillus nidulans contains several group-I introns. Each one has been assayed for its ability to self-splice in vitro in the absence of proteins. The intron from the apocytochrome b gene is unusual among subgroup IB4 introns in being able to self-splice, unlike a similar intron from Saccharomyces cerevisiae. The first intron in the cytochrome oxidase subunit-1 gene self-splices but only correctly completes the first step of splicing; cryptic 3' splice-sites are recognized instead and these are also used at a low frequency in vivo. The highly homologous intron from Podospora anserina completes both steps in vitro. The remaining introns do not self-splice. The correlation between subgroup category, the likely presence of specific tertiary interactions, and self-splicing activity is discussed.
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