These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [The cloning and expression of salmon calcitonin gene in Escherichia coli].
    Author: Di X, Chen S, Song F, Zhang J, Chen W.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1996 Dec; 18(6):423-8. PubMed ID: 9388947.
    Abstract:
    The DNA fragment encoding salmon calcitonin was separated from the salmon DNA with PCR method. It was recombined into the fusion expression vector (pGEX-2T) which included a Glutathion-S-Transferase (GST) gene and was transformed into E. coli JM109 and then expression was induced with IPTG. The fusion protein (GST-sCT) accounted for 38%-40% of the total cellular protein and was purified from the soluble expression products by glutathion sepharose 4B affinity chromatography. The purity of GST-sCT is about 80% and it showed a positive immunological reaction in calcitonin enzyme immunoassay.
    [Abstract] [Full Text] [Related] [New Search]