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  • Title: [Establishment of human amnion cell mutagenesis system by using a shuttle vector pS189].
    Author: Wang H, Yi S, Xiao Y, Li D, Qi J, Fu J.
    Journal: Hua Xi Yi Ke Da Xue Xue Bao; 1996 Dec; 27(4):372-7. PubMed ID: 9389006.
    Abstract:
    Using transient shuttle vector pS189, we established a subclone human amnion cell (FL) mutagenesis detection system, and we detected the mutation specificity of N-methy-N'-nitro-N-nitrosoguanidine (MNNG) in this system. The spontaneous mutant frequency of target gene supF was 1.7 x 10(-5) and MNNG-induced mutant frequency was increased with dosage. The results from 0.8% agarose gel electrophoresis, PCR, PCR-single strand conformation polymorphism (PCR-SSCP) analysis showed that MNNG-induced 89% mutants were point mutants. These results indicated that this system can be used to detect and study the mutagenesis mechanism of potential mutagens.
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