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  • Title: Involvement of afsA in A-factor biosynthesis as a key enzyme.
    Author: Ando N, Matsumori N, Sakuda S, Beppu T, Horinouchi S.
    Journal: J Antibiot (Tokyo); 1997 Oct; 50(10):847-52. PubMed ID: 9402990.
    Abstract:
    The afsA gene of Streptomyces griseus has been postulated to encode a key enzyme for A-factor biosynthesis from primary metabolites commonly present in Streptomyces strains. Escherichia coli cells harboring afsA under the control of the T7 promoter specified distinct A-factor activity in the culture broth, as determined by induction of streptomycin production and aerial mycelium and spore formation in an A-factor-deficient S. griseus mutant strain. Production of the substance(s) having A-factor activity was inhibited by cerulenin, an inhibitor of fatty acid biosynthesis. These observations suggest that afsA encodes a key enzyme in the A-factor biosynthetic pathway in which a beta-keto acid derived from fatty acid biosynthesis and a glycerol derivative serve as precursors.
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