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  • Title: Increased synergistic effect of EGF and IGF-I on DNA synthesis of cultured psoriatic keratinocytes.
    Author: Ristow HJ.
    Journal: Dermatology; 1997; 195(3):213-9. PubMed ID: 9407164.
    Abstract:
    BACKGROUND: DNA synthesis is significantly more stimulated in response to insulin-like growth factor I (IGF-I) in growth-arrested cultures of psoriatic than of nonpsoriatic keratinocytes. Epidermal growth factor (EGF), by itself only a weak stimulator, was recently found to cause a strong synergetic effect when added together with IGF-I to newborn human keratinocytes. OBJECTIVE: (1) To measure this effect in cultured adult psoriatic and nonpsoriatic keratinocytes, and (2) to examine whether the difference in DNA synthesis after IGF-I addition could be due to differences in the binding of this growth factor. METHODS: 3H-deoxythymidine incorporation and 125I-IGF-I binding studies. RESULTS: Psoriatic keratinocytes demonstrate a considerably stronger response to the combination of EGF and IGF-I than nonpsoriatic keratinocytes. This is apparently not caused by differences in binding of IGF-I. CONCLUSION: Differences in the proliferative rate of normal and psoriatic keratinocytes may be in part due to differences in signal transduction distal from the IGF-I receptor or in the cooperation of the EGF and the IGF-I receptor.
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