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  • Title: High-performance liquid chromatographic determination of benomyl and carbendazim residues in apiarian samples.
    Author: Bernal JL, del Nozal MJ, Toribio L, Jiménez JJ, Atienza J.
    Journal: J Chromatogr A; 1997 Nov 07; 787(1-2):129-36. PubMed ID: 9408999.
    Abstract:
    Simple procedures for the extraction and chromatographic determination of benomyl and carbendazim in honey, bees wax, larvae, bees and pollen are proposed. The fungicides were extracted from honey, larvae and bees using ethyl acetate, while methanol was more suitable for wax and pollen samples. Pollen extracts need a further clean-up step with n-hexane. The determination is carried out by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. The procedures have been applied to the analysis of benomyl on honey and larvae samples from hives whose bees were nourished with artificial food mixed with benomyl.
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