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  • Title: Optimization of the separation selectivity of PCBs in a hydroorganic reverse-phase liquid chromatography in the presence of cetyltrimethylammonium bromide with fluorescence detection.
    Author: Mahugo Santana C, Sosa Ferrera Z, Eiguren Fernández A, Santana Rodríguez JJ.
    Journal: Biomed Chromatogr; 1997; 11(6):333-4. PubMed ID: 9413609.
    Abstract:
    The retention of different PCBs of toxicological and environmental interest was studied in a hydro-alcoholic reverse-phase liquid chromatography in the presence of cetyltrimethylammonium bromide (CTAB). The influence of variables like type and percentage of alcohol, concentration of CTAB, temperature and mobile phase flow was determined. According to the aforementioned studies, the appropriate conditions for separation of PCBs in presence of CTAB in a hydro-alcoholic medium are: percentage of 1-Propanol 55% (v/v), concentration of CTAB 5.0 x 10(-3) M, temperature 50 +/- 1 degrees C and mobile phase flow 1 ml min-1.
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