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  • Title: Reorientation of large DNA molecules in concentrated polyacrylamide solution during crossed-field electrophoresis.
    Author: Oana H, Doi M, Ueda M, Yoshikawa K.
    Journal: Electrophoresis; 1997 Oct; 18(11):1912-5. PubMed ID: 9420143.
    Abstract:
    Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120 degree crossed-field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer for larger DNA. Such a backtrack motion has been thought to be an essential motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis.
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