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  • Title: Quantification of apoptotic cells using isolated glomeruli.
    Author: Omori S, Morioka T, Wakasugi M, Kawachi H, Arakawa M, Shimizu F, Oite T.
    Journal: Nephron; 1997; 77(4):474-8. PubMed ID: 9434072.
    Abstract:
    In order to detect apoptotic cells in the kidney, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method is used in tissue sections. But the number of apoptotic cells per glomerulus in several experimental models of nephritides is about 1 per single tissue section even at peak levels. In this study, we have reported that the TUNEL method and immunostaining of cell-specific markers to a whole isolated glomerulus in combination with laser scan microscopy are potentially useful methods for the analysis of cell turnover within glomeruli.
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