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  • Title: Structural analysis of hexa to dodecaoligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Bufo bufo.
    Author: Morelle W, Strecker G.
    Journal: Glycobiology; 1997 Dec; 7(8):1129-51. PubMed ID: 9455914.
    Abstract:
    Hexa to dodecasaccharide-alditols of the jelly coat surrounding the eggs of the toad Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and H-NMR spectroscopy. These highly species-specific carbohydrate chains exhibit new structural features, such as the elongation of the blood group A determinant with an external alpha-1,3-linked galactose unit, or ramification belonging from a fucosylated galactose. The most representative oligosaccharide-alditol of the series was defined as following: [structure in text]. Since the jellies surrounding amphibian eggs are involved in egg-sperm interactions, these structural investigations can provide biochemical support for exploring the fertilization process.
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