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  • Title: Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism.
    Author: Filimonova MN, Gubskaya VP, Nuretdinov IA, Benedik MJ, Bogomolnaya LM, Andreeva MA, Leshchinskaya IB.
    Journal: Biochemistry (Mosc); 1997 Sep; 62(9):983-8. PubMed ID: 9457762.
    Abstract:
    Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclease has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.
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