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  • Title: Substrate and sequential site specificity of cytoplasmic histone acetyltransferases of maize and rat liver.
    Author: Kölle D, Sarg B, Lindner H, Loidl P.
    Journal: FEBS Lett; 1998 Jan 09; 421(2):109-14. PubMed ID: 9468289.
    Abstract:
    The cytoplasmic B-type histone acetyltransferase was purified to apparent homogeneity from maize embryos. We established a novel protocol for easy large-scale preparation of acetylated core histone species, using preparative acetic acid-urea-Triton PAGE. The pure maize histone acetyltransferase B was highly specific for histone H4 under various assay conditions, modifying H4 up to the di-acetylated isoform. Only non-acetylated H4 isoform was accepted as substrate, whereas mono-acetylated H4 could not be further acetylated. The enzyme selectively acetylated lysines 12 and 5 in a sequential manner. The same results were obtained with a partially purified cytoplasmic histone acetyltransferase of rat liver. Protein sequencing results were supported by immunological characterization of acetylated H4 subspecies with site-specific H4-acetyllysine antibodies.
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