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  • Title: Thermostable, high salt-tolerant amylase from Bacillus megaterium VUMB-109.
    Author: Jana M, Chattopadhyay DJ, Pati BR.
    Journal: Acta Microbiol Immunol Hung; 1997; 44(3):281-9. PubMed ID: 9468732.
    Abstract:
    A bacterial strain, Bacillus megaterium VUMB-109, has been isolated and identified which produces salt-tolerant, thermostable amylase (16 U/ml culture filtrate). Cultural conditions such as carbon and nitrogen sources, metal ions, temperature and pH have been optimized for enzyme production. The partially purified enzyme was active over a wide range of pH (4.5-10) and exhibited maximum activity at 95 degrees C, retaining 90% original activity at 100 degrees C. Partially purified enzyme was stable at 70 degrees C for 60 min. The enzyme was stable in NaCl up to 5m over 24 h without losing its original activity.
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