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  • Title: C-terminal regions of D-hydantoinases are nonessential for catalysis, but affect the oligomeric structure.
    Author: Kim GJ, Kim HS.
    Journal: Biochem Biophys Res Commun; 1998 Feb 04; 243(1):96-100. PubMed ID: 9473486.
    Abstract:
    Most microbial D-hydantoinases have been reported to have catalytic properties similar to those of mammalian dihydropyrimidinases. Comparison of the primary structures of microbial D-hydantoinases with mammalian dihydropyrimidinases revealed that the amino acid homology is about 37% and functionally important residues are rigidly conserved at identical positions. Interestingly, however, the C-terminal regions were found to be completely mismatched with each other. In order to investigate the possible role of the C-terminal regions, we deleted the C-terminal regions of the D-hydantoinases from two thermophilic Bacilli and compared the catalytic and structural properties of the mutant enzymes with those of wild-type enzymes. As a result, the C-terminal region was found not to be essential for catalysis, but it does affect the oligomeric structure of the enzyme.
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