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  • Title: Characterization of mannose-labeled glycopeptides from human diploid cells and their growth-dependent alterations.
    Author: Muramatsu T, Koide N, Ceccarini C, Atkinson PH.
    Journal: J Biol Chem; 1976 Aug 10; 251(15):4673-9. PubMed ID: 947903.
    Abstract:
    Mannose-labeled glycopeptides were prepared from human diploid fibroblasts harvested by brief pronase digestion. Combined use of endo-beta-N-acetylglucosaminidase H and D converted most of the mannose-label into arrays of oligosaccharides. They were separated by paper chromatography and were characterized by Sephadex G-25 column chromatography, by affinity column chromatography on concanavalin A-Sepharose, and by successive digestion with alpha-mannosidase and beta-mannosidase. The results indicated that mannose residues existed as clusters of various sizes, which we refer to as "oligomannosyl cores". The large oligomannosyl cores (approximately 7 to 8 mannosyl residues) were predominant in the glycopeptides from growing cells and were preferentially associated with neutral glycopeptides, similar to Unit A glycopeptides of thyroglobulin (mannose-N-acetylglucosamine unit). In glycopeptides from nongrowing cells, the ratio of the large oligomannosyl cores decreased, accompanying the increase of a small oligommanosyl core consisting of 3 mannosyl residues. The small core was preferentially associated with acidic glycopeptides.
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