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  • Title: [RNA typing of granulocyte NA antigens by means of PCR-RFLP analysis].
    Author: Stein EL, Bux J, Santoso S, Kalb R, Mueller-Eckhardt C.
    Journal: Beitr Infusionsther Transfusionsmed; 1994; 32():229-32. PubMed ID: 9480095.
    Abstract:
    An RNA-based method was developed for genotyping of the granulocyte-specific alloantigens NA1 and NA2, mRNA was isolated from neutrophils and reversely transcribed into cDNA. To avoid coamplification of Fc-gamma receptor III-2 in the polymerase chain reaction (PCR), a Fc-gamma receptor III-1 sequence-specific primer was employed. Restriction analysis using Taq I led to NA-specific restriction fragment length polymorphism (RFLP) of PCR-amplified cDNA. The results of the PCR-RFLP typing were in good concordance with serological phenotyping by granulocyte immunofluorescence test and the antigen capture assay MAIGA.
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