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  • Title: [Preparation of human platelets without the use of anticoagulants and study of the effect of Catt on aggregation].
    Author: Lösche W, Hofmann B, Hofmann J, Spangenberg P, Ostermann G, Mindner K, Till U.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1979; 106(5-6):870-8. PubMed ID: 94881.
    Abstract:
    A method for the preparation of human blood platelets is presented which substitutes the use of anticoagulants by a gelfiltration for the removal of plasma calcium from native blood. In a second step the platelets are separated from the gel filtered blood by a centrifugation on a Ficoll density gradient. The anticoagulant-free platelets reveal an intact morphological feature and a normal aggregation behaviour in response to different aggregation inducers. It was found that in ADP-induced aggregation monophasic aggregation is shifted to a biphasic one by increasing concentrations of extracellular calcium.
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