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  • Title: A method for the rapid analysis of neuronal proportions and neurite morphology in primary cultures.
    Author: Isaacs KR, Hanbauer I, Jacobowitz DM.
    Journal: Exp Neurol; 1998 Feb; 149(2):464-7. PubMed ID: 9500959.
    Abstract:
    This article provides basic guidelines for a rapid analysis of subpopulation proportions and neurite morphology in primary cultures. We describe, in E14 mesencephalic primary cultures, an immunohistochemical method for the simultaneous identification of multiple neuronal phenotypes and an estimation of the ratio of subpopulations. In addition, we describe the use of the Renaissance TSA-Direct kit (NEN, DuPont) to enhance the visualization of neurites when the antigen is in low abundance. Finally, a modified sholl analysis is used to rapidly and reliably estimate neurite number and length.
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