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  • Title: The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase.
    Author: Saha SK, Maniscalco SJ, Fisher HF.
    Journal: Biochim Biophys Acta; 1998 Jan 15; 1382(1):8-12. PubMed ID: 9507051.
    Abstract:
    A multiwavelength transient-state kinetic study of the glutamate dehydrogenase catalyzed reaction has proven that an alpha-iminoglutarate complex is an observable intermediate in the reverse direction. It also shows the existence of two enzyme-NADPH-ketoglutarate complexes, only one of which reacts with ammonia rapidly.
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