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  • Title: A modified semi-automated technique for in vitro assessment of encrustation of materials.
    Author: Gepi-Attee S, Feneley RC.
    Journal: Proc Inst Mech Eng H; 1997; 211(6):475-8. PubMed ID: 9509885.
    Abstract:
    In vitro study of encrustation is an important part of assessment of materials as potential alloplasts or devices in the urinary tract. This modified semi-automated technique comprises a circular reaction chamber with an encrustation mixture, the level of which is controlled by a float switch which operates the exit peristaltic pump. The composition of the reactants used simulates infected urine with alkaline pH. Results of a preliminary study of the deposits by scanning electron micrography (SEM) and energy dispersive X-ray (EDX) microanalysis are consistent with struvite and hydroxyapatite, similar to the main minerals deposited on urinary catheters. It is a relatively simple, effective and inexpensive set-up for study of encrustation on materials.
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