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  • Title: Sensitive chiral high-performance liquid chromatographic assay for labetalol in biological fluids.
    Author: Dakers JM, Boulton DW, Fawcett JP.
    Journal: J Chromatogr B Biomed Sci Appl; 1997 Dec 19; 704(1-2):215-20. PubMed ID: 9518153.
    Abstract:
    The four stereoisomers of the combined alpha- and beta-adrenoceptor antagonist labetalol were separated and quantified at therapeutic concentrations by normal-phase high-pressure liquid chromatography using a chiral stationary phase and fluorescence detection. Drug in plasma or urine was recovered by solid-phase extraction with 83+/-5% efficiency. Limits of detection from biological samples (3 ml) were between 1.5-1.8 ng ml(-1). Intra-day and inter-day variation at 25 ng ml(-1) were < or = 2.7% and < or = 5.80% respectively for all stereoisomers. The assay was applied to an examination of the disposition of labetalol stereoisomers after a single oral dose of racemate to a human volunteer. Labetalol appears to undergo enantioselective metabolism leading to relatively low plasma concentrations of the pharmacologically active enantiomers.
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