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  • Title: Calretinin gene expression in the human thalamus.
    Author: Cicchetti F, Lacroix S, Beach TG, Parent A.
    Journal: Brain Res Mol Brain Res; 1998 Feb; 54(1):1-12. PubMed ID: 9526028.
    Abstract:
    The localization and levels of expression of the calcium-binding protein calretinin (CR) in the human thalamus was studied with an in situ hybridization method applied to formalin-fixed postmortem material from normal individuals. The riboprobe used was generated from a specific fragment of human CR cDNA. As visualized on X-ray film autoradiographs, high levels of CR gene transcript occurred in several thalamic nuclei, including the reticular nucleus, mediodorsal nucleus, rostral intralaminar nuclei (paracentral, central medial and central lateral) and several midline nuclei (paraventricular, reuniens and medioventral nuclei). In the reticular nucleus, neurons expressing CR mRNA were few in number but formed dense and widely distributed clusters. In contrast, virtually all neurons in the rostral intralaminar and midline nuclei expressed very high levels of CR mRNA and formed a prominent rim around the mediodorsal nucleus, which contained scattered clusters of labeled neurons. The caudal intralaminar nuclei, principally the centromedian nucleus, displayed very few neurons expressing CR mRNA. Only the medial part of the parafascicular nucleus expressed moderate levels of CR mRNA. The nuclei of the ventral group (ventral anterior, lateral and posterior nuclei) were virtually devoid of CR gene transcript. This highly heterogeneous pattern of mRNA expression suggests that CR may be heavily involved in the function of the so-called non-specific nuclei, but not in that of the specific relay nuclei of the human thalamus. The data also demonstrate that the presence of CR gene transcript can easily be detected on formalin-fixed sections of the human brain.
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