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  • Title: [The detection and isolation of fetal nucleated erythrocytes from maternal blood using flow cytometry].
    Author: Li Z, Xia T.
    Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 1998 Apr 10; 15(2):111-4. PubMed ID: 9531654.
    Abstract:
    OBJECTIVE: To isolate fetal nucleated red blood cells (NRBCs) from maternal blood and to confirm its fetal origin. METHODS: Monoclonal antibody against the glycophorin A (GPA) was used to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully. Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences (SRY) in maternal blood from women carrying male fetuses were detected by polymerase chain reaction (PCR). RESULTS: The PY3.4 position hybridization rates were 31.5%, 0 and 38% respectively in sorted GPA positive cells (GPA+)of 3 samples by FISH, and the results of GPA+ cells of the 3 cases by PCR were positive, negative and positive respectively, which were manifested by the gender after aborticide. CONCLUSION: This study demonstrates that fetal NRBCs are present in the peripheral blood of pregnant women and it is possible to isolate and detect them.
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