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  • Title: Involvement of protein-tyrosine phosphorylation and dephosphorylation in sperm-induced Xenopus egg activation.
    Author: Sato K, Iwasaki T, Tamaki I, Aoto M, Tokmakov AA, Fukami Y.
    Journal: FEBS Lett; 1998 Mar 06; 424(1-2):113-8. PubMed ID: 9537526.
    Abstract:
    We have analyzed tyrosine-phosphorylated proteins in Xenopus laevis eggs before and after fertilization by immunoblotting with anti-phosphotyrosine antibody. A number of egg proteins with different subcellular distribution became tyrosine-phosphorylated or dephosphorylated within 30 min after insemination. Tyrosine kinase-specific inhibitors genistein and herbimycin A were found to inhibit sperm-induced egg activation judged by the egg cortical contraction. Surprisingly, sodium orthovanadate, a tyrosine phosphatase inhibitor, also inhibited the egg activation. Moreover, we found that fertilization-dependent tyrosine dephosphorylation of 42-kDa mitogen-activated protein kinase was inhibited in genistein-treated eggs. These results suggest that both protein-tyrosine phosphorylation and dephosphorylation pathways play an important role in the sperm-induced Xenopus egg activation.
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