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  • Title: Flow cytometric method to detect lymphocyte transformation in drug-allergic hepatic injury.
    Author: Ikeda T, Noguchi O, Kobayashi F, Tozuka S, Tokushima K, Sakamoto S, Marumo F, Sato C.
    Journal: Dig Dis Sci; 1998 Mar; 43(3):513-20. PubMed ID: 9539645.
    Abstract:
    Flow cytometric methods for the analysis of incorporated bromodeoxyuridine are extremely rapid and simple. We investigated whether these methods were useful for detecting drug-allergic hepatic injury in 18 patients with drug-allergic hepatic injury, 18 healthy controls, and 9 nonallergic patients receiving drugs. Peripheral blood mononuclear cells were stimulated with drug solutions. Incorporation of bromodeoxyuridine was detected after labeling with FITC, and S-phase cells were counted by flow cytometry. Percentages of S-phase cells in drug-stimulated culture minus those in spontaneous cultures were less than 1% in both healthy controls and nonallergic patients receiving drugs. Taking 1% as the upper limit, 13 patients (72%) were judged as positive. After the in vitro addition of interleukin-2, two patients among five who had been judged as negative were judged as positive. Lymphocyte transformation test by flow cytometry may be useful in the diagnosis of drug-allergic hepatic injury.
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