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  • Title: Nuclear factor of activated T cells (NFAT) as a molecular target for 1alpha,25-dihydroxyvitamin D3-mediated effects.
    Author: Takeuchi A, Reddy GS, Kobayashi T, Okano T, Park J, Sharma S.
    Journal: J Immunol; 1998 Jan 01; 160(1):209-18. PubMed ID: 9551973.
    Abstract:
    The molecular basis of the immunomodulatory properties of 1alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3) remains elusive. We demonstrate here that 1alpha,25(OH)2D3-mediated suppressive effects on the inducible expression of cytokine genes in human T cells may, in part, be due to diminished activity of the transcription factor NFAT. The vitamin D3 receptor (VDR) and its heterodimeric partner retinoid X receptor alpha (RXR alpha) specifically bound to the distal NFAT site in the human IL-2 promoter, and this binding was abolished by mutating unique regions in the NFAT oligonucleotide. In vitro inhibition of NFAT complex formation was noted when VDR-RXR alpha heterodimers were added to DNA binding reactions containing nuclear extracts from activated B or T cells, whereas in vitro NFkappaB complex formation was not significantly influenced. Furthermore, 1alpha,25(OH)2D3 treatment of activated T cells resulted in decreased formation of NFAT complexes detected upon incubation of nuclear extracts from these cells with 32P-labeled probe. Transient expression of both VDR and RXR alpha, but not of a single component, was capable of inhibiting expression of a NFAT-driven reporter gene in stimulated jurkat cells in a ligand-dependent manner. These results suggest that NFAT plays a crucial role in 1alpha,25(OH)2D3-mediated immunosuppressive activity.
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