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  • Title: Purification and characterization of lipopolysaccharide induced TNF-like factor from rabbit serum.
    Author: Lee TH, Rhim TY, Son OK, Kim YH, Kim SS.
    Journal: Immunol Lett; 1998 Feb; 60(2-3):97-102. PubMed ID: 9557950.
    Abstract:
    A TNF-like factor was purified from lipopolysaccharide (LPS) induced New Zealand white rabbit serum. The TNF-like factor was purified by DEAE-Sephacel, Sephacryl S-200, Mono-Q, CM-affi gel Blue, Superose 12 H/R preparative columns to the specific activity of 4 x 10(6) U/mg protein. The purified protein was 45 kDa in its oligomeric form and 22 kDa in its monomeric form. Rabbit TNF-like factor had a pI value of 5.0 and was resistant to trypsin digestion. The TNF-like factor reacted with polyclonal-Ab against human TNFalpha on immunoblot and immunoprecipitation analysis and interacted with human TNF receptors. Taken together, rabbit TNF-like factor might be a high molecular weight form of rabbit TNFalpha.
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