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  • Title: A simple fluorometric assay for type B monoamine oxidase activity in rat tissues.
    Author: Suzuki O, Noguchi E, Yagi K.
    Journal: J Biochem; 1976 Jun; 79(6):1297-9. PubMed ID: 956156.
    Abstract:
    A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4] activity towards beta-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10 mg to 100 mg of liver tissue in 3 ml of reaction mixture for up to 90 min of incubation. Using this method, the V max values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).
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