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  • Title: [Number of apolipoprotein(a) kringle IV repeats and mobility of isoforms in PAGE].
    Author: Abe A, Takashima M.
    Journal: Rinsho Byori; 1998 Mar; 46(3):283-8. PubMed ID: 9564769.
    Abstract:
    Serum apo(a) isoform sizes were determined by SDS-polyacryl amide gel electrophoretic (PAGE) method, followed by a high sensitive immunoblotting. The relation between the number of apo(a) kringle IV encoding sequences in the apo(a) gene, as assessed by pulsed field gel electrophoresis and genomic blotting (genotype), and the apo(a) isoforms (phenotype) in SDS-PAGE was evaluated in 78 individuals. The correlation of apo(a) allele number between phenotype and genotype method was high (r = 0.513) in 178 expressed apo(a) alleles. This nomenclature is achieved by using standard serum of kringle IV repeats. We conclude that this method is an approach to genotyping to designate the phenotypes by kringle IV repeats.
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