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  • Title: [Preparation of lymphotoxin and properties of it].
    Author: Lebedev LR, Pustoshilova NM, Sinichkina SA, Andreeva IS.
    Journal: Prikl Biokhim Mikrobiol; 1998; 34(1):120-6. PubMed ID: 9566293.
    Abstract:
    The process of isolation of highly purified human lymphotoxin was studied and optimized. A set of methods providing an increase in the content of the target product in the biomass (plasmid DNA amplification and selection of clones of transformed cells) was applied at the stage of cultivation. A two-step purification scheme (ion-exchange chromatography on DEAE-Biotone A1 and hydroxylapatite) was developed. A number of physical characteristics were studied.
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