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  • Title: An improved method for chemical deglycosylation of gastric mucus glycoprotein.
    Author: Paszkiewicz-Gadek A, Gindzieński A, Porowska H.
    Journal: Rocz Akad Med Bialymst; 1997; 42(1):18-25. PubMed ID: 9581460.
    Abstract:
    We describe an improved method for chemical deglycosylation of gastric mucin which involves: reduction, alkylation, desialylation, periodate oxidation with beta-elimination and two-steps of TFMSA/anisole treatment. The product was 96% deglycosylated protein with amino acid composition similar to purified mucin with apparent molecular weight of 90 kDa.
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