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  • Title: [Immobilization of Bacillus subtilis proteases on a styrol copolymer with maleic anhydride with covalent and coordination bonds].
    Author: Slobodianikova LS, Latov VK, Belikov VM.
    Journal: Prikl Biokhim Mikrobiol; 1979; 15(2):262-8. PubMed ID: 95830.
    Abstract:
    Insoluble enzymes have been obtained through an interaction of the complex preparation of proteases, protosubtilin G15x, with the sterol and maleic anhydride copolymer in aprotic dipolar solvents and as a result of formation of mixed enzyme-polymer complexes of protosubtilin G15x and the sterol and malic acid copolymer in aqueous solution in the presence of metal ions. The effect of metal ions on the yield and activity of the resultant immobilized preparation has been studied.
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