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  • Title: [Endonucleolysis of chromatin in rat thymus and spleen lymphocytes after irradiation].
    Author: Solodushko VO, Matyshevs'ka OP, Kucherenko NIe.
    Journal: Ukr Biokhim Zh (1978); 1997; 69(4):88-94. PubMed ID: 9583129.
    Abstract:
    The level of soluble DNA fragments (polydesoxynucleotides) in thymus and spleen lymphocytes is enhanced 12 h after the whole-body X-ray irradiation in dose of 0.5 and 1 Gy. The level of Ca2+, Mg(2+)-DNAase activity in lysed cells extracts, measured with calf spleen DNA, was enhanced in the region of 0.1-0.5 mM CaCl2 in incubation medium and decreased at 5 mM CaCl2. Monitoring of DNA fragmentation by electrophoresis in agarose gels showed the presence of fragments with approximately 200, 400, 800 bp length. X-ray induced DNA fragmentation is supposed to be associated with Ca2+, Mg(2+)-endonuclease activation.
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