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  • Title: Codon reading properties of tRNA variants substituted within the anticodon loop.
    Author: Ouchi R, Takai K, Yokoyama S, Takaku H.
    Journal: Nucleic Acids Symp Ser; 1997; (37):115-6. PubMed ID: 9586026.
    Abstract:
    Effects of base substitution within tRNA anticodon loop on the codon reading activities were quantitatively analyzed with the use of a set of unmodified tRNA molecules with GGA anticodon. The first (position 32) and the last (position 38) nucleotides of the anticodon loop of the wild-type molecule was changed from C32A38 to U32A38, U32G38, and C32G38. The codon reading activities of these variants relative to that of the wild type molecule were measured in a cell-free translation system. The reading of both the UCU and UCC codons were lower in all the three variants than in the wild-type molecule.
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