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  • Title: [The usefulness of a selective disk-broth media for the detection of group B streptococci in the vagina].
    Author: Bosch J, Murillo S, Rico M, Salgado M.
    Journal: Enferm Infecc Microbiol Clin; 1998 Feb; 16(2):83-4. PubMed ID: 9586366.
    Abstract:
    BACKGROUND: The detection of pregnant group B streptococci (GBS) carriers allows intrapartum administration of antibiotic prophylaxis to these women and prevents perinatal infection by this microorganism. The aim of this study was to determine the usefulness of a selective media to detect GBS in the vagina based on the disk-broth method. MATERIAL AND METHODS: One thousand six hundred five vaginal swabs were seeded in blood agar with colistin and nalidixic acid (NDA) and in a selective disk-broth tube consisting of 2 ml of Mueller-Hinton broth with 5% serum and a disk of 30 micrograms of amikacin which was reseeded at 24 hours of incubation in blood agar with NDA. RESULTS: GBS was isolated by either of the two methods in 209 samples (13.0%); in 160 (9.9%) in the initial dish of blood agar with NDA and in 205 (12.7%) in the subculture of the selective disk-broth media. CONCLUSIONS: The selective disk-broth media used in this study is a simple method which allows detection of GBS in vaginal samples of women with GBS cultures in a negative agar blood dish.
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