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  • Title: Effect of enamel microabrasion on bacterial colonization.
    Author: Segura A, Donly KJ, Wefel JS, Drake D.
    Journal: Am J Dent; 1997 Dec; 10(6):272-4. PubMed ID: 9590914.
    Abstract:
    PURPOSE: To determine if microabraded surfaces inhibit Streptococcus mutans colonization. MATERIALS AND METHODS: Ten extracted molars were obtained and sectioned in half mesio-distally. The mesial side was treated with microabrasion (PREMA Compound). The distal surface was designated as the control site. A plastic tube was secured on each surface with resin composite, exposing a surface of 13.2 mm 2. S. mutans 10,499 was cultured in TSB-YE supplemented with 5% sucrose and 2.5% glucose was added to each secured site in addition to 50 microliters of cell suspension. Samples were incubated in a 5% CO2 chamber for 7 days at 37 degrees C. Samples were taken from each site, incubated with dextranase (75 micrograms 1 ml), sonicated, and vortexed to disrupt cellular aggregates as much as possible. Samples were then plated on Mitis-Salivarius agar using a spiral plating system. Enumeration of numbers of colonizing bacteria was achieved following standard spiral plating system methodology. RESULTS: The mean for the microabraded group was 1.12 x 10(2) cfu/tooth and for the control it was 1.15 x 10(4). Analyzing the data revealed a significant difference (P = 0.0188).
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