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  • Title: Multiprimer--PCR for screening of IgH and T-cell receptor rearranged genes in acute lymphoblastic leukemia.
    Author: Xu B, Zhou S, Sun J.
    Journal: Chin Med J (Engl); 1997 Feb; 110(2):100-3. PubMed ID: 9594278.
    Abstract:
    OBJECTIVE: To develop a multiprimer-polymerase chain reaction (multiprimer-PCR) method for detecting immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) rearranged genes in the same amplification. METHODS: Multiprimer-PCR protocol, a mixture of four different primers used in the same reaction detected IgH CDR-III and TCR V gamma I-J gamma Ligenetic rearrangements in 40 acute lymphoblastic leukemia (ALL) patients. RESULTS: Thirteen cases were found to have IgH CDR-III and TCR V gamma I-J gamma genetic rearrangements. Ten cases were found to have TCR V gamma I-J gamma genetic rearrangements. Thirteen cases were found to have IgH CDR-III genetic rearrangements. The amplification result of multiprimer-PCR was the same as separate amplification. The sensitivity of multiprimer-PCR was 10(-4)-10(-5) DNA level, which was the same as separate PCR amplification. All positive cases with IgH rearranged gene were confirmed by Southern blot. CONCLUSION: Multiprimer-PCR could screen two distinct rearranged genes in a single amplification, and is more suitable for screening of specimens collected at diagnosis.
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