These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Application of PCR-SSCP technique in detection of rpoB gene mutation in rifampin-resistant Mycobacterium tuberculosis].
    Author: He X, Zhuang Y, Li G.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 1996 Dec; 19(6):338-41. PubMed ID: 9596814.
    Abstract:
    OBJECTIVE: To evaluate rpoB gene mutation in rifampin-resistant Mycobacterium tuberculosis and its relationship with rifampin resistance. METHODS: Forty clinical isolates of Mycobacterium tuberculosis were analyzed by PCR-SSCP technique, with H37Rv reference strains as control group. RESULTS: The sensitivity of amplication products of 411bp and 258bp were found to be 5 pg/microliters, 500 organisms per milliliter and 1 pg/microliter, 500 organisms per milliliter respectively. rpoB gene belongs to genus specificity. Characteristics of SSCP graph of 258bp fragment: Ten sensitive strains were the same as H37 Rv. Thirty strains of rifampin-resistant or multidrug resistance, including rifampin, were different from H37Rv except for three strains. Positive rate was 90%, while specificity 100%. CONCLUSIONS: Results showed that PCR-SSCP technique could detect rPOB gene mutation, which might associate with rifampin resistance and be helpful to rapid detection and research of rifampin-resistant Mycobacterium tuberculosis.
    [Abstract] [Full Text] [Related] [New Search]