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  • Title: Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active.
    Author: Bahl CP, Wu R, Narang S.
    Journal: Gene; 1978 Apr; 3(2):123-34. PubMed ID: 95988.
    Abstract:
    A 17-nucleotide-long synthetic DNA molecule constituting the minimal recognition sequence of the lactose operator has been cloned in E. coli using the vehicle pBR313 and a synthetic HindIII adaptor. The clones containing the lac-pBR313 hybrid DNA constitutively produced beta-galactosidase. The level of beta-galactosidase was high and comparable to that obtained in cells carrying a 21-nucleotide-long synthetic lac operator on pMB9 plasmid or cells carrying a natural lac operator on pOP203-1 plasmid.
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