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  • Title: DNA curvature in solution measured by fluorescence resonance energy transfer.
    Author: Tóth K, Sauermann V, Langowski J.
    Journal: Biochemistry; 1998 Jun 02; 37(22):8173-9. PubMed ID: 9609713.
    Abstract:
    The sequence-induced curvature of DNA fragments free in solution was characterized by measurements of the end-to-end distance using fluorescence resonance energy transfer (FRET). The 31 bp oligonucleotides were labeled at their 5' ends with fluorescein as the donor and rhodamine X as the acceptor. We compared a curved oligonucleotide with three phased A6 blocks and a control containing (AT)3 instead of the A6 blocks. The increased efficiency of energy transfer of the A6-containing DNA indicates the existence of a permanent sequence-induced curvature, the magnitude of which is in good agreement with estimates from theoretical curvature predictions. Energy transfer efficiency and correspondingly curvature increases with NaCl concentration.
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