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  • Title: [Novel site-specific endonucleases from Brevibacterium species].
    Author: Sokolov NN, Korolev SV, Rina M, El'darov MA, Gervaziev IuV, Skriabin KG, Bouriotis V.
    Journal: Mol Gen Mikrobiol Virusol; 1998; (2):35-8. PubMed ID: 9611761.
    Abstract:
    New site-specific endonucleases BecAI and BecAII have been detected in Brevibacterium species A. Endonuclease BecAII free from contaminating nonspecific endonucleases, exonucleases, and phosphatases was isolated by column chromatography on phosphocellulose, heparin sepharose, and DNA cellulose. It recognizes and cleaves the 5'-GG decreases CC-3' sequence and is a true isoschizomer of HaeIII restriction enzyme. The other restriction endonuclease, BecAI, cleaves Ad2 DNA at least by 2 sites but not the DNA of phage lambda, T7, SV40, phiX174, and plasmides pBR322 and pUC19. The substrate specificity of BecAI indicates its appurtenance to the super rare restriction endonucleases.
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