These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Glucose-induced pulsatile insulin release from single islets at stable and oscillatory cytoplasmic Ca2+.
    Author: Bergsten P.
    Journal: Am J Physiol; 1998 May; 274(5):E796-800. PubMed ID: 9612236.
    Abstract:
    The cytoplasmic Ca2+ concentration ([Ca2+]i) and insulin release were measured simultaneously in mouse pancreatic islets cultured overnight. [Ca2+]i was 105 nM and insulin release 3 pmol.g-1.s-1 at 3 mM glucose. An increase to 7 mM glucose reduced [Ca2+]i transiently, whereas insulin release doubled and was pulsatile with a frequency of 0.47 min-1. [Ca2+]i oscillations with similar frequency appeared at 11 mM glucose associated with increased amplitude of the insulin oscillations, raising the secretory rate 10-fold. In the presence of 16 and 20 mM glucose [Ca2+]i was > 300 nM and showed no oscillations apart from two islets, which demonstrated [Ca2+]i oscillations with small amplitude at 16 mM glucose. Insulin release with maintained frequency increased by 46 and 31%, respectively. When the glucose concentration was increased from 3 to 11 mM, [Ca2+]i decreased with a nadir that appeared significantly earlier than when the glucose concentration was raised from 3 to 7 mM. Glucose-induced insulin release from the isolated islet is pulsatile both at stable and oscillatory [Ca2+]i, with changes in secretory rate caused by the sugar also when [Ca2+]i is unchanged.
    [Abstract] [Full Text] [Related] [New Search]