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  • Title: Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves.
    Author: Franck SM, Bosworth BT, Moon HW.
    Journal: J Clin Microbiol; 1998 Jun; 36(6):1795-7. PubMed ID: 9620426.
    Abstract:
    A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.
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