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  • Title: An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
    Author: García-Patrone M, Tandecarz JS.
    Journal: Cell Mol Biol (Noisy-le-grand); 1998 May; 44(3):557-61. PubMed ID: 9620454.
    Abstract:
    Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buffer of pH 9 was described. In the present report three additional protein complexes with different degrees of stability and the effect of that dissociating sample buffer are described. The study of SDS critical micellar concentration values as a function of pH explains the improvement of dissociating properties at pH 9.
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