These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Age-dependence of the lateral diffusion coefficient of Con-A receptor protein in the skeletal muscle membrane of C57BL/6J mice.
    Author: Zs-Nagy I, Tanaka S, Kitani K.
    Journal: Mech Ageing Dev; 1998 Apr 01; 101(3):257-68. PubMed ID: 9622229.
    Abstract:
    The lateral diffusion coefficient (Dp) of the Con-A receptor protein was measured in the sarcolemma of the quadriceps femoris (QF) muscle of male and female C57BL/6JNia mice in four age groups between 2 and 26 months. Freshly prepared, ex vivo taken muscle strips were stained with Con-A-FL conjugate for 10 min, and fluorescence recovery after photobleaching (FRAP) measurements were carried out on 20-30 cells per animal, at 37 degrees C. Using this technique, Dp, and the fractional recovery (mobile fraction = FR%) of these proteins can be measured. In the youngest male and female age groups, Dp values of 5.72E-10 and 5.43E-10 cm2/s, and FR% values of 43.3 and 36.3%, were found, respectively. Dp displayed a characteristic, significant, negative, linear correlation with age in both sexes. The slope of the linear regression line calculated per month of age was 1.06E-11 and 0.96E-11 cm2/s for males and females, respectively; both of them differ from zero highly significantly. FR% values tended to increase slightly with age, yet the estimated average Dp = D(FR), calculated for the total Con-A receptor pool, maintained its significant, negative, linear age-correlation. The physiological significance of these changes needs to be clarified in the future.
    [Abstract] [Full Text] [Related] [New Search]