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  • Title: Determination of ciprofloxacin in plasma and urine by HPLC with ultraviolet detection.
    Author: Kamberi M, Tsutsumi K, Kotegawa T, Nakamura K, Nakano S.
    Journal: Clin Chem; 1998 Jun; 44(6 Pt 1):1251-5. PubMed ID: 9625049.
    Abstract:
    A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. The quantification limit was 0.01 mg/L in plasma and 0.5 mg/L in urine. This method was sufficiently sensitive for pharmacokinetic studies.
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