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  • Title: Thermophilic strain Bacillus species AA contains several site-specific endonucleases.
    Author: Alzhanov DT, Alzhanova DV, Zheleznaya LA, Matvienko NI.
    Journal: Biochemistry (Mosc); 1998 May; 63(5):537-45. PubMed ID: 9632889.
    Abstract:
    Thermophilic strain Bacillus species AA contains several site-specific endonucleases and three of them have been identified. BspAAI recognizes the sequence 5'-C downward arrow TCGAG-3' and cleaves it after the first "C" forming 4-nucleotide 5'-ends and is an isoschizomer of XhoI. BspAAII recognizes the sequence 5'-T downward arrow CTAGA-3' and cleaves it after the first "T" forming 4-nucleotide 5'-ends and is an isoschizomer of XbaI. BspAAIII recognizes the sequence 5'-GGATCC-3' and is an isomer of BamHI. The optimal temperature and pH values are 42-48 degreesC and 7.0-8.0, respectively.
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