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  • Title: Transcription factor NF-E2 is essential for the polyploidization of a human megakaryoblastic cell line, Meg-J.
    Author: Kobayashi S, Teramura M, Ito K, Iwabe K, Inaba T, Mizoguchi H.
    Journal: Biochem Biophys Res Commun; 1998 Jun 09; 247(1):65-9. PubMed ID: 9636655.
    Abstract:
    Transcription factors regulating the process of megakaryocyte development remain largely unclarified. To clarify them further, we used a human megakaryoblastic cell line, Meg-J, which showed prominant polyploidization and augmented platelet glycoprotein (GP) Ib expression after incubation with thrombopoietin (TPO, c-mpl ligand) and K252a (an indolocarbasole derivative). Under these conditions, we analyzed the expression of the transcription factors and observed that the expression of NF-E2 p45, but not those of GATA-1, GATA-2, Tal-1/SCL, Evi-1, and MafK, was increased after TPO and K252a stimulation. Gel-shift assay confirmed the enhanced binding activity to the NF-E2 site. The abolishment of NF-E2 p45 with NF-E2 antisense oligomers inhibited TPO plus K252a-induced polyploidization. These findings suggest that NF-E2 p45 is essential for the polyploidization of megakaryocytic cells.
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