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  • Title: [Effects of herpes simplex virus thymidine kinase gene transduction and prodrug on ovarian cancer cell].
    Author: Liang Z, Kuang J, Lin Z.
    Journal: Zhonghua Fu Chan Ke Za Zhi; 1997 Aug; 32(8):467-70. PubMed ID: 9639739.
    Abstract:
    OBJECTIVE: To evaluate the efficacy of gene therapy with the herpes simplex virus thymidine kinase (HSV-TK) gene transference followed by ganciclovir (GCV) therapy in ovarian cancer. METHODS: HSV-TK gene was inserted into the hind III site of pLNSX vector and recombinant plasmid (pLNS/HSV-TK) was constracted. Then, the recombinant plasmid was transfered into PA317 packaged cell and the producer cell line of recombinant retrovirus vector pLNS/HSV-TK was established. The cytotoxicity efficacy of HSV-TK/GCV system to AO cell line was evaluated by microcucture tetrajolium test (MTT) method. RESULTS: The recombinant retroviral vector pLNS/HSK-TK can transfer HSV-SK gene into the genome of AO cell line and make it sensitive to GCV. The growth inhibitory rate of AO cell line transfered by pLNS/HSV-TK was 98.0% in the medium containing 400 mumol GVC. CONCLUSION: The ovarian cancer cell line transfered by HSV-TK gene regressed rapidly in response to the GCV therapy.
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